Enzyme-linked Immunoabsorbent Assay (ELISA)

The epidermis is a tissue with a high metabolism and is subject to constant regeneration.
If the stratum corneum is injured (long- or short term) or infected, messenger substances are released (e.g. Biomarkers). Beyond these second messengers is Interleukin-1α (IL-1α), which in turn triggers additional inflammational factors (e.g. IL-6 and IL-8). The IL-1α can be extracted using the skin washing method and quantified using the ELISA technique, and thus is a marker for inflammation.
The variation of the ELISA technique employed for our purposes is known as the Sandwich ELISA method, and comprises two antibodies being bonded specifically to the antigen to be verified. A subsequent enzymatic reaction leads to a colour change, which can be quantified by photometric measurement.

Schematic presentation ELISA
The first antibody is fixed to the well chamber (A). The Interleukin-1α binds to the first and second antibody (B-C). After addition of the streptavidin-HRP (D) and the substrate (E), an enzymatic reaction takes place, which convert the substrate to a blue colour. The reaction is stopped by adding the stop solution. The colours turns into yellow.



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